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Journal: PLOS Biology
Article Title: Heme acts as a metabolic brake on erebosis in the Drosophila gut
doi: 10.1371/journal.pbio.3003942
Figure Lengend Snippet: (A) Feeding of CYP P450 inhibitors 1-Aminobenzotriazole (1-ABT) or piperonylbutoxide (PBO) does not restore the inhibition of erebosis by heme. 1-ABT with Ho RNAi and PBO with Mrp5 RNAi result in >50% class0 and no class2, which is similar to Ho RNAi or Mrp5 RNAi with DMSO. (B) ROS levels are not increased by Mrp5 RNAi . Using hsFLP system, Mrp5 RNAi was induced only in GFP+ cells. ROS probe dihydroethidium (DHE, magenta) is not changed in GFP+ Mrp5 RNAi cells (dashed line). (C) Ho RNAi or Mrp5 RNAi does not increase signals of lipid peroxide probe Liperfluo (green), while bleomycin increases them. (D) Feeding of a reducing agent N-acetylcysteine (NAC) does not increase erebosis. P values were calculated using Fisher’s exact test (A, D) and adjusted with the Benjamini–Hochberg method (D). Scale bars, 50 μm.
Article Snippet: Flies were fed with standard food with 15 μM
Techniques: Inhibition